612 Proteomic and secretomic comparison of young and aged fibroblasts highlights cytoskeleton as a key component during aging

Crucial for skin homeostasis, synthesis and degradation of extracellular matrix components are orchestrated by dermal fibroblasts. During aging, alterations component expression, such as collagens enzymes, lead to reduction the mechanical tension defects wound healing. The aim this study was better understand molecular underwent fibroblasts during aging comparing secretomic proteomic signatures from young (<35 years) old (>55 donors, using LC-MS/MS. Samples were analyzed a Q-Exactive Plus coupled Nano-LC Proxeon 1000 (Thermo Scientific). Data analysis done label-free quantification on Progenesis QI Proteomics (Waters). comparison secretome revealed significant alteration in expression 16 proteins resting condition, 11 after 24h-lasting TGF-β1-stimulation (fold change>2, p-value <0.05), with 77% decrease number secreted aged cells. Proteome between showed change 63 73 TGF-β1-stimulated interest, 67% increase Noteworthy, majority differentially-expressed molecules belongs actin-related cytoskeleton-associated linked migratory contractile functions human Altogether, our present data describe an age-related fibroblast protein secretion age conversely enhancement more than 60% cytoplasmic accumulation, actin ECM organization two main fields modified aging.